|
KMID : 0545120070170091533
|
|
Journal of Microbiology and Biotechnology
2007 Volume.17 No. 9 p.1533 ~ p.1537
|
|
|
Continuous Production of Lactosucrose by Immobilized Sterigmatomyces elviae Mutant
|
|
Lee Jong-Ho
Lim Jung-Soo
Park Chul-Hwan
Kang Seong-Woo
Shin Hyun-Yong
Park Seung-Won
Kim Seung-Wook
|
|
|
Abstract
|
|
|
|
In this study, in order to develop a continuous production process of lactosucrose in a packed-bed reactor, Sterigmatomyces elviae ATCC 18894 was selected and mutated. The mutant strain of S. elviae showed 54.3% higher lactosucrose production than the wild type. Reaction conditions such as temperature, pH, substrate concentration and flow rate were also optimized. Under optimized reaction conditions (50oC, pH 6.0, 25% sucrose and 25% lactose as substrate, flow rate 1.2 ml/min), the maximum concentration of lactosucrose (192 g/l) was obtained. In a packed-bed reactor, continuous production of lactosucrose was performed using S. elviae mutant immobilized in calcium alginate, and about 180 g/l of lactosucrose production was achieved for 48 days.
|
|
|
KEYWORD
|
|
|
Continuous production, lactosucrose, packed-bed reactor, Sterigmatomyces elviae mutant, whole-cell immobilization
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|